FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718768
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37460.21794680841160644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1506.382252E-721.0023561
GTATAAC1408.247156E-620.0022451
CCGTTTA1055.469299E-419.9993369
GTGTAAA4400.018.2975081
GTATCAT4450.018.08928566
GTGTAGC4300.017.9089871
GTATAAG2952.3646862E-1117.7986091
CGTATCA4350.017.70107765
GTATAGA1803.656647E-617.5019651
GTCCGTA1000.00968600917.5019631
GTGTAAG3950.016.8373321
GTACCGA1250.001761750116.8018861
GTATTAT2108.5784086E-716.6685371
TCTGTCG5500.016.5449058
GTCTAGC3401.0913936E-1116.4724371
GTCTTGC11350.016.3454461
GTACTAG1300.002289701516.1556591
ATCATTA4800.016.04113468
GTATTGG2854.940375E-915.9667041
TACACTG4850.015.875765
Produced by FastQC (version 0.11.5)