FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114135
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG1403.2893877E-722.50018970
TCTGTCG6100.020.0807118
TATACTG3850.020.0030065
GTCTTGC12350.019.8415091
GTATAGG3100.019.196891
GTGTAAG5350.018.9752661
GTATAGA3600.018.4754371
ATCTCGT5700.018.42120770
GTCTCGC6700.017.7642861
TATCTCG7400.017.50014970
GTGTAGA5450.017.3424681
CCGATCA1250.001763991816.798959
GTGTTAG8500.016.4734541
GTATTGG3900.016.1566561
GTATATA4400.015.911861
TCGCTCT7050.015.8889144
TGTCGCC7350.015.71330410
GTCGCCC7800.015.25581811
GTATAAC2302.214072E-615.220041
TTACACT7150.015.1771084
Produced by FastQC (version 0.11.5)