FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442363
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6200.031.047748
CTGTCGC7900.023.9235539
TGTCGCC7700.023.18133710
TCGCTCT6700.022.9860084
TCGCCCA8300.021.50557712
GTCGCCC7650.021.04531711
GTCTCGC7300.019.660751
GTCTTGC14900.019.4998321
GTGTTAG9550.019.427261
GTATTAA4900.018.5744651
CGCTCTG7600.017.9612585
GTATTAG4700.017.8752611
GTCTTAC6800.017.5028631
GTATAGA4400.017.5028611
CTCGCTC7500.017.2677213
GTATAGC2852.6739144E-1017.1957931
TCTCGCT7750.017.1630422
GTATTCG1452.4376891E-416.8993151
TACACCG1250.001762880716.8006845
GTGTTAC6150.016.5067631
Produced by FastQC (version 0.11.5)