FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963055
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAAAGATATCTCGTATGCCGT39460.2010132166444649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG8650.029.53644470
AAGGGGG10100.025.29606469
AAAGGGG11900.022.05798168
AAAAGGG12400.020.886367
GTATAAG3200.019.6927681
GTACGAA1651.5902478E-619.0960161
CTATACT1852.2682252E-718.9234984
GTGTATA3900.017.953521
AAAAAGG15500.017.6122366
GTATTAG2005.1406823E-717.504681
TGTATAA3002.910383E-1117.49933210
TATAAGA3002.910383E-1117.49933212
CTTGAAA17050.017.03703757
GTACTAA1653.389882E-516.9742371
GGGTAAG3104.9112714E-1116.9400141
GTCCTAT3005.529728E-1016.3377021
GTATTAA1957.821387E-616.1581691
GTGTAGA4350.016.0962581
TTGAAAA18050.016.09315758
TCTGTCG4800.016.0410528
Produced by FastQC (version 0.11.5)