FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014342
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10550.025.8830811
GTCTTGC17450.023.8740121
TCTGTCG7900.023.480898
TCGCTCT10000.022.0502514
GTACTCG1656.791561E-821.217291
CTGTCGC10000.020.6498939
TGTCGCC9700.020.2060810
CGCCGTA1801.7043385E-719.4453168
CTCGCTC11350.019.1197953
GTATTAC4450.018.8810041
TCTCGCT11050.018.6910972
GTATTAG5450.018.6283911
TAGTTCG950.007212778618.42126366
TCGCCCA10100.017.67317612
GTCCTAC5950.017.6513581
GTACTAT3800.017.5042651
CGTGTTA3255.456968E-1217.23067910
GTCGCCC9700.016.95867311
CGCTCTG11400.016.8861565
GTACTAC3401.0913936E-1116.4746021
Produced by FastQC (version 0.11.5)