FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2505195
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7100.020.7035248
GTCTTGC14900.019.7348141
GTCTCGC7400.018.4490011
GTATAGG3650.018.2221971
GTGTTAG9750.017.9516931
TCGCTCT6900.017.7565654
GTGTAGG7500.017.7362731
TACCCGT1200.001339998717.503255
GTATACG1200.001340178117.50291
GTATAAC2802.0736479E-1017.50291
GTATTAC2451.3276804E-817.1456971
ATCTCGT7800.017.05138470
GTATAGT3501.8189894E-1217.0028171
GTATAGC3501.8189894E-1217.0028171
CTGTCGC8250.016.9691229
GTGTAAA9100.016.925881
GCGCGAT5450.016.69601416
GTATAGA4900.016.4312931
GTGTATA6200.016.373681
GTATTAT4500.016.336041
Produced by FastQC (version 0.11.5)