FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646594
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATCG1900.031.31499770
TCTGTCG6650.025.2625168
TACACCG1102.9216242E-522.2763735
CACGAGC9600.021.50987270
TATCTCG9200.021.30380870
TGTCGCC8350.020.1196310
CTGTCGC9250.019.6755499
TCGCTCT8550.019.6523384
GTATAAG4800.018.9610771
GTCTTAC5550.018.9216561
GTCGCCC7650.018.75804711
GTCTCGC9050.018.5662211
TAGTCCG950.007206219718.4240675
GTGTTAG10200.018.1889061
TAACACG3700.017.9759144
GTCTTGC15300.017.8457181
GTCTACG1200.001340393617.5025331
TAGTGCG1000.00969502317.4995567
GTGTAAG7950.016.7319811
TCGCCCA9450.016.66624512
Produced by FastQC (version 0.11.5)