FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979570
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7500.028.9341358
TGTCGCC10300.022.76762410
CTGTCGC9800.022.5006269
GTCGCCC10100.021.83082411
GTATTAG5250.021.3350011
TCTCGTA5850.020.94040370
GTCTCGC7900.019.4951931
GTACTAT4000.019.2515031
GTATTAT5050.018.7143341
TCGCCCA11800.018.38879812
GTATAAT5600.018.1264171
GTATAGA4650.018.0659281
TATCTCG9150.017.9783470
CACGAGC7600.017.96072470
GTCTTGC17100.017.8084091
TCGCTCT9750.017.2332734
GTCTCAC22500.016.8013131
GTATTAC4400.016.705851
TAATACT5900.016.6125834
TTATATG8300.016.44596570
Produced by FastQC (version 0.11.5)