FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979570
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8450.023.613581
TCTGTCG7550.021.7874898
GTCTTGC16450.020.85471
GTACCGT850.003788332220.5918251
TCGCTCT9350.018.7173274
GTATTAT5500.018.4577621
GTCGCCC8400.018.33283611
GTACGAG1150.001007351118.2640531
TGTCGCC9100.018.07643310
GTGTTAT6500.017.772331
CTGTCGC9650.017.771549
GTGCTAC6600.017.503051
GTATAGG4000.017.503051
GTATTAC4500.017.1140941
CTCGCTC10150.016.8980793
TACACCG1452.4402139E-416.8972285
GTATCGG1250.001761428316.8029291
CGCTCTG10000.016.4506595
TCTCGCT10250.016.3917262
GGTATAC4150.016.0268921
Produced by FastQC (version 0.11.5)