FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976888
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6150.031.3080751
GATACCG500.007210562528.0008325
TCTGTCG6300.027.7771958
TCGCTCT6700.026.1201764
GTCGCCC6100.024.67161411
TGTCGCC7450.023.95922910
CTCGCTC6950.023.6715623
CTGTCGC7500.023.3328469
TCTCGCT7950.022.8978142
GTATAAG3750.022.40521
CGCTCTG7600.020.7242985
CGGTACT1205.719057E-520.417274
TCGCCCA7400.019.86444912
TAACACG2353.8016879E-1019.3622764
CCCGATT1107.478236E-419.09147519
GTCTTGC11700.018.5513131
ACCCGAT1150.001008337818.2609518
GTATTAT4150.017.7149521
TATACGA1000.009693935517.49963411
GTATTAG4450.017.3073841
Produced by FastQC (version 0.11.5)