FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365840
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7200.026.734568
CTGTCGC9050.022.042929
TGTCGCC8850.020.95923610
GTCTCGC8250.019.5222741
TCGCTCT9150.019.5140764
GTACCGG1107.461986E-419.0978761
GTATTAA4100.018.787341
GTATAGA4100.018.787341
GTCGCCC8200.018.77939811
GTATAGG3750.018.6734771
ATACGGT950.007201152418.4262146
GTCTTGC13050.018.2442021
TCTCGCT9400.017.8777282
CGCTCTG9400.017.877355
GTATTGC3600.017.5063861
GTATAAG4000.017.5063861
TAGACCG1000.009677689517.5049045
TACACCG1000.009677689517.5049045
CCGATTG1000.009690653517.50083536
TCGCCCA8900.016.90913212
Produced by FastQC (version 0.11.5)