FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365840
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6800.025.7354648
GTCTTGC14100.021.5986271
GTCTCGC7000.021.5028691
TGTCGCC8200.020.48750910
CTGTCGC8800.020.2842259
GTATTCG1601.1859811E-619.6901281
TGTCGAC1601.1876637E-619.68721610
GTACTAC2705.456968E-1219.447041
ATAACGG900.00527763719.4449843
TCGCTCT7800.019.2954
GTCGCCC7750.018.96746611
GTGTTAG9400.018.6195051
TAGTACT3200.018.5938744
TATAACG950.007212525718.4211752
CTGTCGA2104.2775355E-818.3334549
GTATATA5200.018.1755031
GGGTACG1351.4117986E-418.1505721
GTATTAG4550.017.694671
GTATAGC2601.4606485E-917.5023371
GTGTTAT6500.017.233071
Produced by FastQC (version 0.11.5)