FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332884
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA604.986715E-429.16580214
TCTGTCG5700.025.7887088
TGTCGCC7350.023.80881710
TAGAACG750.00183162523.3376434
CTGTCGC7300.022.5335799
GTCTCGC6600.021.7464391
GTCTTGC14650.020.0719661
GTCGCCC7250.019.79209111
GTATATA6650.019.477281
GTATAAG4000.019.2535531
TCGCTCT6300.018.8923764
TTTACGC950.007205984618.4240593
CGTTTCG1351.4135535E-418.147619
GTATAAC2551.1277734E-917.8464321
TCGCCCA7650.017.84222412
GTGTTAC6150.017.6455361
GATACGA1200.001340162817.5028572
GGATACG1452.4372358E-416.8996721
GTATTAG5200.016.830031
GGATAGA6050.016.7799591
Produced by FastQC (version 0.11.5)