FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658774
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11000.034.405611
TCTGTCG9900.029.8151538
TCGCTCT12450.029.4904254
TCTCGCT12700.026.6454662
GTCTTGC25000.026.4923171
GTGTTAC10500.025.6466711
GTGTTAG19800.025.3631081
CTCGCTC13700.025.2260883
GTCTTAC10100.025.221171
CTGTCGC12500.024.7654849
GTACTAG5350.024.4870951
GTCTTAT10250.024.1420211
CGCTCTG14200.023.8281155
GTATTAT8600.023.6961691
GTATAGA7400.023.604681
GTATTAG8750.023.289951
GTATAAT7500.023.289951
GTATTAA7550.023.1357121
GTGTAGG13050.022.3083781
GTACTAC5001.8189894E-1221.8343281
Produced by FastQC (version 0.11.5)