FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2658774
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9550.040.116021
TCTGTCG8800.033.5373578
TCGCTCT12100.030.3477754
TCTCGCT12750.029.362592
CTCGCTC13000.028.8006323
GTGTTAG15500.028.5192071
GTATAGT7300.027.2495161
GTGTACG3254.1836756E-1127.2029361
CTGTCGC13350.026.9663779
GTCTTGC20250.026.195421
CGCTCTG14000.025.2005545
GTATAAG8050.024.7107431
GTGTAGG14500.024.388841
GTATAGA7700.023.920331
GTTAGCC12700.023.8115463
GTCTTAC8700.023.711371
GTATAGG7300.023.2125511
GAGTGCA61450.023.0766096
GGAGTGC57050.022.5912065
GTCTAGG15600.022.196841
Produced by FastQC (version 0.11.5)