FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561131
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.039.7840088
GTATTAG8350.038.3852961
GTCTCGC10950.035.258221
GTCTTGC23250.033.5242841
TCGCTCT11500.032.5520484
CTGTCGC13450.031.5793069
GTATTAT9450.027.7504061
TCTCGCT13250.026.6274382
GTCTTAC9400.026.3481241
TTATACT7850.025.6780014
CTCGCTC13800.025.5661983
CGCTCTG13550.024.970745
GTCCTAT8150.024.1326391
GTGTAAC7900.023.9742431
TTGCTCT31500.023.7681624
TATACTG8400.023.139685
GTACTAC5400.022.9326271
CTTGCTC33000.022.909973
GTCTCAC30650.022.8159961
GTATTGC6400.022.7640041
Produced by FastQC (version 0.11.5)