FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719015
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.043.760268
GTCTTGC24850.035.147721
GTCTCGC11100.034.753231
CTGTCGC12900.034.0430569
TCGCTCT11850.034.0212364
GTGTTAC10600.031.5859721
CTCGCTC13300.027.6118053
TCTCGCT14300.026.6880052
GTATAAT8400.025.9946671
GTGTTAG18700.025.6888481
GTATTAC6050.025.2642361
ACCGTGT8000.025.1974418
CGCTCTG14350.025.0840995
TTCACCG7750.025.0808645
GCGTAGT3251.4224497E-924.6349491
TTGCTCT37500.024.5730514
CTTGCTC34050.024.3196323
CCGTGTT9150.022.8173569
GTACGGC1600.002127029522.7453331
GTCTCAC30750.022.4864441
Produced by FastQC (version 0.11.5)