FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719015
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9800.043.336278
CTGTCGC13500.033.0670439
GTCTTGC24400.032.303921
GTCTCGC11800.031.8382661
GTACTAG5000.029.4660051
GTATAAC5300.029.1880261
GTATAAG7600.029.0782951
TTACACT14850.028.1225684
TCGCTCT12800.026.4387724
GTATAAA10500.025.2565751
TTGCTCT36550.024.624994
GGTATAC5750.024.3414821
TACACTG17900.024.135245
TCTATCG1500.001558634823.9940918
GTCTTAC10500.023.1518611
GTCTCAC31950.023.0563411
TCTCGCT15100.022.888942
GTATTAC7150.022.6661591
CGCTCTG14050.022.549135
GTATTAG10200.021.6661821
Produced by FastQC (version 0.11.5)