FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742131
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG800.00210764835.999895
TCTGTCG11150.032.9218258
GTCTTGC24450.031.0795561
GTCTCGC11900.029.7584761
CTGTCGC14150.026.460739
GTGTAAC8700.025.4400791
GCGTAGG3152.3846951E-823.4210221
GTCTCAC30000.022.378791
TTGCTCT34450.022.153784
TCTCGCT14350.022.076992
GTATAGG7100.021.8211381
GTGTAGG14550.021.8032821
GTCTTAC9650.021.4065741
GTATAAG7600.021.3562761
GGGTTTC46300.021.3520831
TCGCTCT12850.021.2917634
GTGTTAA11200.021.0789221
CTCGCTC12700.020.9763153
GGAGTGC56350.020.699145
GTATTAA8600.020.5887131
Produced by FastQC (version 0.11.5)