FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458300
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG657.590675E-444.3105965
GTCTCGC10000.030.2608931
TCTGTCG8550.026.0979468
GTCTTGC23500.024.8117331
CTCGATC8500.022.8641788
CTCGCTC13700.022.6000213
TCGCTCT12750.022.5897164
CCTATCG1600.002264742322.5010199
CTGTCGC11550.022.4425749
GGTATAC4950.022.3657741
TCTCGCT13900.022.2739352
GGAGTGC51050.021.8623525
GTGTTAC9850.021.7299961
GTATTAT8500.021.7079581
TATTCCG2003.2520507E-421.6014185
GAGTGCA56900.021.3788916
GAGTAAT7600.021.3651981
GTACTAG5201.8189894E-1221.2904971
TCGATCT9500.021.2220129
TCTCGAT9500.021.2151057
Produced by FastQC (version 0.11.5)