FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885556
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10300.040.5384948
CTGTCGC14400.030.9960279
GTCTCGC12150.029.9927521
GTCTTGC27750.028.1023981
GTGTTAG18650.025.7921581
GTATTCG1550.001754771923.5104471
TCGCTCT12600.023.4251634
GTATAGG6850.023.407481
TCTGTCA34250.023.3313148
GTGTAGA13850.023.153971
TCTCGCT14850.022.7889062
GTGTTAT11550.022.0855711
GTATAAG8250.022.0855711
GTCTTAC11600.021.9903761
GTCTCAC36100.021.8041481
GTATTAT8700.021.7809431
GTATTAG10050.021.7559361
GTACACG4409.094947E-1121.5334321
GGGTTTC47700.021.3910561
GGTATAC5550.021.0111371
Produced by FastQC (version 0.11.5)