FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630923
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10300.042.635648
CTGTCGC13100.034.621789
GTCTCGC11650.031.2894541
GTATAGC5400.028.3517231
GGTATAC6200.028.2210711
TCGCTCT11200.027.6384774
GTCTTGC25450.026.6409111
GTATAAG6750.024.841511
GTATTAC6500.024.6753461
GTATTGA6750.023.7614441
GTCTCAC32400.023.4014231
GTCTTAC12250.022.6152531
GTATTAA8750.022.4962251
GTGTAGA14300.022.4321331
GTGTAAC8500.022.300181
GTGTTAG18050.022.2146471
GCTACGC1952.7400695E-422.1558073
TACACCG2603.4106579E-622.1503355
GTTTCAC40150.021.7005523
GAGTGCA65050.021.6906416
Produced by FastQC (version 0.11.5)