FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642493
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12300.036.1311451
GTCTTGC23500.031.932021
TCGCTCT12700.031.7449424
CTCGCTC13750.030.3737013
TCTGTCG9800.030.1195148
TCTCGCT14650.029.4907672
CGCTCTG13550.027.6283055
GTGTAAC8600.027.1086921
GTATAGA7800.026.1529371
CTGTCGC12450.025.4433069
GTATTAT10200.024.9991321
GTCTTAC11350.024.3918511
GTACTAT6150.023.6925541
GTATAGG6700.022.8350281
GTATAAG8450.022.4168051
TCTGTCA34550.022.2959488
GTGTACG3004.3514774E-721.8563821
GTATTAC6700.021.7476461
GTCTCAC30850.021.7264981
GTGTAAG12350.021.236971
Produced by FastQC (version 0.11.5)