FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642493
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10050.030.7966618
GTCTTGC25350.029.6685641
GTCTCGC11100.028.564051
GGTATAC5500.028.1534021
GTGTAAC7900.027.0673181
CTGTCGC14200.025.861139
GCGCTAG1751.1202934E-425.2806071
GTATAAC4450.024.8545281
TCGCTCT11100.024.6514
GTACACG2101.3654355E-524.578371
GTATTAA9100.024.3082771
GTCTTAC10950.023.5682981
GTATAGC6650.023.2847711
GTATTAG8900.023.197561
GGAGTGC59000.022.944565
GAGTGCA64750.022.2318216
GTCTCAC29700.022.0957051
GTGTAAG13750.021.986471
TTGCTCT36400.021.9581384
GTATTAC6400.021.890111
Produced by FastQC (version 0.11.5)