FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314100
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10800.039.0590321
TCGCTCT12400.038.3191684
TCTCGCT12750.035.5948372
GTCTTGC20350.035.0250631
CTCGCTC13050.034.2245563
TCTGTCG8900.033.165598
CGCTCTG12600.032.568535
CTGTCGC10750.030.1368549
GTACACG2155.9626836E-727.0625511
TTGCTCT26550.024.9469324
GGCGTAA2101.5184027E-524.2435381
GGAGTGC54750.023.8006235
GTCTAAC4650.023.4614871
GAGTGCA59000.023.4284256
CTTGCTC26700.022.663383
TACCCCG2552.8796858E-622.5862265
TCTGTCA28750.022.286718
TCGGAAC1650.002700434521.8294473
CTGGAGT65100.021.7992693
ACCGTGT6950.021.753468
Produced by FastQC (version 0.11.5)