FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314100
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9300.038.7087448
TCCGGTA1051.9314587E-434.2856252
CTGTCGC11150.032.2869159
GTCTTGC19600.030.8570631
GTCTCGC9200.029.7390561
TCGCTCT8900.028.3139234
TTGCTCT28150.025.8324154
GTCTCAC24350.024.8377191
GAGTGCA55050.023.0185186
GGAGTGC50950.022.6098585
GGGTTTC34600.022.4739321
CGCTCTG11450.022.0082025
GTCTTAC7900.021.8733631
GTTTCAC32050.021.7904243
TACACTG12950.021.6828755
GTTCTAT6050.021.4214341
GTGTTAG13200.021.272671
TATCCCG2404.60608E-520.9994935
TTATACT5850.020.9225714
GTCCTAG9650.020.8911381
Produced by FastQC (version 0.11.5)