FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2744586
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11300.035.0448761
TCTGTCG11250.033.919998
TCGCTCT13000.031.5692214
GTCTTGC26350.028.6912171
GTATTAG9950.028.2216131
CTGTCGC14500.027.806899
GTGTTAC10950.027.6169341
GTGTTAG18650.027.0246141
CTCGCTC14200.026.8732323
CGCTCTG13350.026.426965
GTACTAT6300.026.2861861
GTAACGC1658.8297864E-526.181813
GTATAAT8550.025.2636131
GTACTAG5900.024.4072171
GTATTAC6550.024.1836411
GTGTAAC10200.024.0004311
TAATACT8450.023.8579834
TCTCGCT14650.023.5908682
GTGTAAA17550.023.3850351
TTGCTCT38450.022.8452474
Produced by FastQC (version 0.11.5)