FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024065
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12500.038.0128568
GTCTCGC13050.036.7756161
GTCTTGC28700.034.7108461
CTGTCGC14900.031.889989
TCGCTCT15300.031.0562554
TCTCGCT14800.029.2094762
CTCGCTC15750.027.4476343
CGCTCTG17350.027.3867865
GTAATAC6300.025.1603323
GGTATAC6850.024.4153961
GTGTAAC8350.024.3836121
TTGCTCT40050.024.2676584
GTCTCAC38700.023.6747881
GTATTAG10550.023.4343531
CTTGCTC38100.023.0711423
GTGTAGG17800.022.8767621
GTGTTAC13150.022.6717281
GTCTTAC11400.022.324911
GTTTCAC42450.022.2345243
TATACTG9550.021.8620685
Produced by FastQC (version 0.11.5)