FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882458
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13900.037.295561
TCGCTCT14000.036.51364
GTCTTGC22200.035.3519171
CTCGCTC16100.031.7509563
TCTGTCG12850.031.376848
TCTCGCT16550.029.5830382
CTGTCGC16700.028.8856859
GTCTCAC27750.027.503141
CGCTCTG15700.026.5982255
GTATTAG9000.026.4004211
TTACACT12000.026.3995044
TTGCTCT31500.025.370954
GTCTTAC9700.025.2375161
GTACTCG2601.2254895E-724.9234711
GTCTAGC9750.023.6311441
TCTATCG1550.001883837223.225378
GTACAGA10800.022.6670271
GTATAAA9850.022.6602571
ATAGCGT1952.7421486E-422.1534296
CTTGCTC31650.022.0659353
Produced by FastQC (version 0.11.5)