FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125654
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG800.002107824735.999435
TCTGTCG13400.029.5517738
GTCTTGC29000.028.5526451
GTCTCGC11850.027.9502681
GTCTTAC11000.024.873531
CTGTCGC16800.023.5710589
GTATTAC7400.023.35211
TCGCTCT12800.023.0621364
TAATACT9000.022.3996474
GTGTTAC12950.022.2400991
GTATCGG1650.002708242321.8188861
TACCCGT2654.018755E-621.7355066
GGGTAAT10400.021.4622291
GTACTAT8200.021.073851
TCCGTAC2755.539414E-620.9451243
GGGTAAG14850.020.8491551
GTATTGC7600.020.8427771
GTCTCGA25950.020.808926
GTGTTAG21800.020.808011
TATCGGC1750.003809473720.572092
Produced by FastQC (version 0.11.5)