FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772120
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.038.897348
GTCTCGC11850.035.5926861
TCGCTCT13100.032.424874
CTGTCGC12800.032.0599179
GTCTTGC25250.029.0878121
TCTCGCT14750.026.8637082
CTCGCTC14300.025.6938633
GTATAGA8550.023.8145831
GTATTAC6750.023.7011831
GTCTTAC10150.023.6428031
CGCTCTG14950.023.5967625
GTATTAA8450.023.2358281
GTATAGC5450.022.6831551
GGCTAGA16700.022.643211
TTACTCT15000.022.5581844
GTGTTAG18100.022.4988611
GTGTTAC12000.022.4218561
GTACAGG15350.022.2659151
GTACTAT6650.021.8705351
GGAGTGC59650.021.8456865
Produced by FastQC (version 0.11.5)