FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772120
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT11750.031.8635644
TCTGTCG9750.031.7535788
GTCTCGC11450.031.4407841
GTACTAT5700.027.7892381
GTATTAG8900.027.5053881
GGTATAC6150.025.755881
GTCTTGC25100.024.9559671
GTGTTAG18550.024.8407611
GTCTAGC11350.024.739881
CGCTCTG13700.024.7005235
TAATAGT6850.024.174984
TCTCGCT13700.023.6494372
GTCTTAC10800.023.3331381
CTGTCGC13000.023.2613439
TAATACT7500.023.0398064
GTACTAA6900.022.956331
GTATTCG1952.7419193E-422.153661
CTCGCTC14500.021.8480933
TGTTAGC16300.021.643992
CCGTGTT8450.021.3015989
Produced by FastQC (version 0.11.5)