FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725374
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12150.040.29298
GTCTCGC12300.037.2239151
CTGTCGC13850.036.906649
TCGCTCT14650.031.4512774
GTCTTGC26400.029.4555031
TCTCGCT15350.028.1636892
CTCGCTC15550.027.3380973
GGTATAC5600.027.2532221
GTATAGA9350.027.2046431
CTATACG1358.4239815E-426.6644214
CGCTCTG16700.025.4349845
GTGTAAA16750.025.1651671
GTACTAG5000.024.7095891
GTATTAT9250.024.3560311
TTGCTCT34250.023.5425424
GTATAAC5250.023.5329421
GTCTCAC30650.023.4742281
TAATACT8900.023.4586984
CTTGCTC32700.023.3563373
GTATAAT8600.022.8166521
Produced by FastQC (version 0.11.5)