FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725374
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11850.038.2798231
TCTGTCG11200.032.1422128
GTCTTGC26300.031.210221
TCGCTCT15000.028.7994254
TCTCGCT15950.027.5370142
CTGTCGC14450.027.4047999
CTCGCTC15250.026.9109363
GTGTTAC11450.026.4114071
GTGTTAG19450.025.1731191
GTACTAG6350.024.9457551
TAATACT8800.024.5449644
TTGCTCT37300.023.7421514
GTATTAG10150.023.4096891
CGCTCTG18200.023.340195
GTGTAAA16700.022.85111
GGCTAGA17300.022.4747771
AATGGCG13700.022.0725525
GTCTTAC9500.021.9797171
GTATAGG7900.021.8741841
GTTTCAC42900.021.8177473
Produced by FastQC (version 0.11.5)