FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190822
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10700.036.6905821
TCTGTCG8700.034.755348
CTGTCGC10500.031.5398859
GTCTTGC18650.029.2366121
GTATTAA5550.027.5087551
GTACTAG4300.027.0518021
TCGCTCT11600.026.6871364
ACTGTCG2508.390816E-825.9175578
GTGTAAC6750.024.7724361
CTCGCTC12300.024.6088833
TCTCGCT13250.024.4762212
GTGTAAA12200.024.4325431
GTTTCAC30050.024.2228163
CGCTCTG12850.024.0911145
GTATAGA6350.024.0430851
GTGCTAC8500.023.94881
GTGTTAG14600.023.9019321
GGGTTTC35550.023.5181351
TTGCTCT25600.023.0603264
GTATTGG7350.022.7501951
Produced by FastQC (version 0.11.5)