FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190822
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.041.645888
GTCTCGC11000.036.001451
GTCTTGC21200.035.661811
GTATTAC3850.031.7934881
CTGTCGC12950.031.1342549
TCGCTCT12050.030.4721684
GGTATAC4450.029.124771
TCTCGCT13400.027.9407842
GTGTTAG13150.027.925081
CTCGCTC12550.027.5370733
GTATTAT5250.027.4296761
GTCTTAC7700.027.1179751
TAATACT5500.026.1810784
CGCTCTG13050.024.8268875
TACGACC1450.001277621324.8268854
GTCTCAC25600.024.1884731
CTAATAC6300.023.9993233
TCTGTCA30000.023.5193378
GTACAAT7050.023.4903071
GTATTAG6450.023.4428021
Produced by FastQC (version 0.11.5)