FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278418
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9800.037.1185721
TCTGTCG7500.034.5571338
TCGCTCT10900.031.7037934
TCGATCT8950.030.5672979
GTCTTGC21100.029.3078381
TCTCGAT9500.028.7976117
CTGTCGC10900.028.4013149
CTCGATC9750.028.0592128
CGCTCTG11500.027.5455445
GTCTCAC25650.027.5126061
GTCTCGA20250.026.6644556
TCTCGCT11900.026.0323582
CTCGCTC12250.025.8766843
GTGTAGA11600.025.0870341
GTATATA9200.024.5143971
TCGAACT13400.024.17719
GTATAAT6350.024.0598491
TACTGCG2101.642915E-523.998017
GAGTGCA57950.022.7349576
GGTCTCG21550.022.7173755
Produced by FastQC (version 0.11.5)