FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979776
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8200.039.5072368
GTCTCGC10500.039.3942221
CTGTCGC9700.037.8509189
TCGCTCT10750.035.525524
TCTCGCT10800.034.6947362
CTCGCTC10350.033.41753
TATACCG1402.8721022E-530.87125
CGCTCTG10900.028.4166095
GTATTAG6900.028.3962961
GTCTTGC18550.027.7754731
GGCGAAC1606.7877794E-527.2131141
GTTCGAC1100.0099675126.2023533
GTGTTAC8400.025.9172531
GTCTCAC22750.025.8375071
GTGTAAA12450.025.6466311
GTATAGC4250.025.6123451
GCGTAGT2001.0583923E-525.3989091
GTATTAA6750.024.726981
GGGTTTC34750.024.2242411
GTATAAC3301.7553248E-924.1894361
Produced by FastQC (version 0.11.5)