FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029637
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8900.046.100298
GTCTCGC9600.040.3974761
CTGTCGC11250.039.029789
TCGCTCT9700.038.5868844
GTCTTGC18000.032.1147231
GTGTACG2558.0035534E-1131.5647321
TCTCGCT11950.031.3408742
CGCTCTG11600.031.0255955
CTCGCTC11000.028.7910423
GTATTAT5100.028.6952111
GTGTAAC6000.028.0495681
GTATACG1050.00737040627.8753451
GGCTAGA11750.026.1553781
GTCTCAC21700.025.6273371
GTATTAG6000.025.6104761
GTAGTAC3409.822543E-1125.4038623
GTGTAAA12100.025.3988191
GTATATA6950.024.2154541
GGTATAC3952.0008883E-1124.0821841
GTCTTAC8050.023.6334461
Produced by FastQC (version 0.11.5)