FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997209
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.046.6970568
CTGTCGC11200.042.4234439
GTCTCGC8900.038.3578261
TCGCTCT10200.036.024514
GTCTTGC16000.035.8635521
CGCTCTG10250.033.0255285
CTCGCTC10400.031.8678363
GTGTAAA11050.029.579881
TCTCGCT10850.027.8913422
TTCACCG5700.027.7986435
GTACCGT1050.00796223427.4376226
TAGTGCG1050.00796223427.4376227
GTGTTAG13200.026.9630071
GTATTAT6000.026.6328491
GTCTCAC22950.025.9523871
GAGTGCA52350.025.1773596
GGAGTGC46900.024.7245735
TTGCTCT23350.024.6848894
GTCCTAC6650.024.0296361
GTGTTAC8250.023.7714671
Produced by FastQC (version 0.11.5)