FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997209
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8300.052.904288
CTGTCGC10550.041.6224179
GTCTCGC9050.037.203581
TCGCTCT10350.034.0787734
GTCTTGC17850.032.3939821
TCTCGCT11700.030.1624642
CTCGCTC11900.029.6391983
GTATTCG1254.875996E-429.27761
TAATACT5650.026.7546374
GTATTAG6350.026.5112111
CGCTCTG11500.026.289345
GGGTAAC6450.026.1001871
GTATTAA5750.025.4587821
GTTAGCC10150.024.8210033
GTGTTAG13000.024.7733541
GTGTAAT8050.024.549541
GGAGTGC45050.024.4469595
GTGTAAC6650.024.2145541
GGGTTTC29950.024.1943441
TTGCTCT26050.023.7639684
Produced by FastQC (version 0.11.5)