FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085739
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8500.042.3486448
GTCTCGC9100.038.2779121
GTCTTGC18700.034.926041
TCGCTCT11300.033.1548164
TCTCGCT11500.029.44712
CTGTCGC12000.029.3970159
GTATTAG5450.029.2937241
GTCGTAA1000.0060479529.027421
CTCGCTC11900.028.455923
GGTATAC3600.028.22111
GTCTTAC8000.027.2132071
CGCTCTG11750.026.9718975
GTATAGA5400.026.877241
GTGTTAG11900.026.832071
GTATTAT5450.026.6306571
GTATAGT5000.026.1246781
GTTTCAC27400.025.506033
GTACATA5450.025.2991261
GTATTAA5750.025.2412321
ACTGTCG2601.2265446E-724.9205468
Produced by FastQC (version 0.11.5)