FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447672
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.044.2493368
CTGTCGC12600.037.7106069
GTCTCGC10500.035.9451871
TCGCTCT11550.035.5515174
GTATTAG7050.032.9448551
GTCTTGC23000.032.503951
CTCGCTC12750.032.2054863
TCTCGCT13100.031.3463212
CGCTCTG12650.031.3140875
GGTATAC4000.030.8471951
GTCTTAC9450.027.6501461
GTGTTAA11050.027.587591
CGTTTCG1050.00797920427.4258969
GTATTAC5850.027.2956561
GTATTAA6500.026.7993721
GTATAGG6450.025.8818241
GTCTCAC29650.025.4586491
GTCTAGA10850.025.4202961
TTGCTCT29700.024.7405264
GTATAAC4700.024.708641
Produced by FastQC (version 0.11.5)