FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447672
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9750.037.65268
GTCTCGC11150.034.77591
GTCTTGC21750.031.9552171
TCGCTCT11050.030.6171654
CTGTCGC14050.029.7153669
CGCTCTG13400.025.7849245
GTACTAG4000.025.606221
GTACTCG2651.2622877E-724.8470081
GTCTTAC9500.023.8734681
GTATAAC4600.023.856731
TCTCGCT14300.023.6679062
GTCTCAC27850.023.6425741
GTGTAAC5950.023.3622171
GTATTAG6650.023.1033571
GTGTTAG14400.022.8626981
GGTATAC5200.022.5109631
GAGTGCA63350.022.4982136
GTTTCAC33700.022.4274523
GTATAGG6300.022.0643181
GGCGTAA2002.9201116E-421.9481891
Produced by FastQC (version 0.11.5)