FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448466
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9750.040.6035778
GTCTCGC10250.034.9984321
GTCTTGC19550.033.3287961
CTGTCGC13500.030.924349
TCGCTCT11400.030.3063584
ATACCGT950.00489885530.3063566
TAATACT5550.029.8285524
GTATTAA6100.028.8043331
CGGACCG1255.378882E-428.791045
CGCTCTG12700.027.7708845
GTACGCC1306.7657325E-427.6836933
GTATAGG6300.025.565751
GTATAAG6600.025.512931
GTGTTAG14150.024.317441
TCTCGCT13550.023.9191422
GTACACG2502.0958832E-623.4275251
GGAGTGC54300.023.19725
GAGTGCA58650.023.0721056
CTCGCTC14900.022.7043443
GTATTAG6900.022.2816121
Produced by FastQC (version 0.11.5)