FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199525
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9400.040.585938
GTCTTGC18650.037.275171
GTCTCGC9050.036.386371
CTGTCGC11750.033.6947489
GTATAAT6400.033.1583441
TCGCTCT11350.031.0761894
GTATTAG6800.029.055591
TCCGCGT1255.376522E-428.793048
GTCTTAC7950.028.5344431
GGGTTAT6000.026.8315811
TCTCGCT11950.026.516112
GTACTAC3900.026.2686821
GGGTTTC35950.026.054691
GTGTTAG15200.025.9971051
TTGCTCT27850.025.8465354
TTCACCG6200.025.5422125
CTCGCTC13000.025.4701863
CGGTACT1701.083301E-425.4056224
GGTATAC4101.8189894E-1224.9872821
TAATACT6350.024.9388524
Produced by FastQC (version 0.11.5)