FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2183516
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.050.569948
CTGTCGC11700.042.4567769
GTCTCGC10700.034.590361
TCGCTCT10350.034.1104744
GTCTTAC8800.032.9872441
GTCTTGC20950.030.8300781
CGCTCTG11750.028.1944055
TCTCGCT12550.028.1315942
CTCGCTC12150.027.8710693
GTATTAT5800.027.5272851
GTATTAA6150.027.140721
GTATTAG6650.026.1913741
GTCTCAC25450.024.2381691
TTGCTCT28500.023.5109484
GTATTAC4950.023.4575941
GACGCTA1550.001884714523.2232028
TTACTCT12150.023.1270564
GTGTTAG14500.023.022821
GTTTCAC31550.022.8366643
GGGTTTC34350.022.6061041
Produced by FastQC (version 0.11.5)