FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2183516
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8100.046.211718
GTCTCGC8550.039.356181
TATATCG951.0735314E-437.886125
GTCTTGC17100.036.789471
TCGCTCT9550.033.1652344
CGCTCTG10450.030.9977345
CTGTCGC13500.028.7941119
CTCGCTC11000.028.7934513
TCTCGCT10550.028.672092
GTGTAAC6350.027.6476861
GTATTAA7250.026.233521
GGATACG1709.709956E-525.8180581
GGGTACG2101.4520356E-524.3837221
GTAACGC1801.5966527E-423.9945433
GGTATAC4600.023.853641
GTATTAG6800.023.6665531
TTGCTCT26950.023.504864
GTCTCAC26800.023.4738831
GTGTTAG12850.023.3400611
TCTGTCA26250.023.3089858
Produced by FastQC (version 0.11.5)