FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379326
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8300.036.429538
GTCTCGC9600.035.5492481
GTCTTGC20600.034.5432051
GTATTAA6600.033.0051041
TCGCTCT11450.032.719674
TAATACT5750.031.3244324
CTCGCTC12600.028.5897583
TCTCGCT12250.028.2321282
GTATTAC5050.027.3190771
GTATTAG7800.026.996481
GTATAAG6450.025.8923741
CTGTCGC11900.025.4088339
CGCTCTG13150.025.1939955
GTACTAT5800.025.0383551
GTCTCAC26700.024.7476481
GTACTAC4151.8189894E-1224.4953541
GTACGCA2101.5378682E-524.2037411
GTCTTAC8850.023.793511
GTGTAGT12450.022.7456861
GCGTAAT1600.002156719822.6910081
Produced by FastQC (version 0.11.5)