FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379326
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9750.041.34528
CTATGCG1355.260172E-737.3263139
GTCTTGC22850.036.834881
GTCTCGC9400.035.8160441
GTATTAG6900.032.8821531
TCGCTCT11900.032.6654364
GTATTCG1702.7997212E-630.1367741
CTCGCTC11950.030.1173253
CTGTCGC13500.029.3278149
TTGCTCT31650.029.1123944
TCTCGCT12600.028.0041472
CTTGCTC29550.027.2819143
GTATAGG5700.026.964481
GTATTAT7100.025.7708841
GTGTAAA12350.025.4829161
GTCTCAC27750.025.3195441
CGCTCTG14300.025.169565
GGGTTTC36300.025.0013051
CTATACT6350.024.9396654
GTCTTAC9800.024.6453781
Produced by FastQC (version 0.11.5)